Premium
The significance of lipid accumulation in breast carcinoma cells through perilipin 2 and its clinicopathological significance
Author(s) -
Kuniyoshi Shimpei,
Miki Yasuhiro,
Sasaki Akari,
Iwabuchi Erina,
Ono Katsuhiko,
Onodera Yoshiaki,
Hirakawa Hisashi,
Ishida Takanori,
Yoshimi Naoki,
Sasano Hironobu
Publication year - 2019
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12831
Subject(s) - perilipin , lipid droplet , breast cancer , oil red o , pathology , immunohistochemistry , lipid metabolism , breast carcinoma , medicine , cancer , intracellular , biology , endocrinology , microbiology and biotechnology , adipose tissue , mesenchymal stem cell , lipolysis , adipogenesis
Both systemic and intratumoral lipid metabolism have been recently reported to play pivotal roles in both tumor development and progression in various human malignancies including breast cancer. However, its details have remained largely unknown in breast cancer patients. Therefore, in this study, we focused on perilipin 2, which is involved in constituting the intracellular lipid composition. Perilipin 2 was first immunolocalized in 105 cases of breast cancer. The status of perilipin 2 immunoreactivity was significantly positively associated with histological grade, Ki‐67 labeling index and HER2 status and negatively with estrogen receptor status of these patients. Subsequent in vitro study also revealed that its mRNA expression in triple negative breast carcinoma cells was higher than cells of other subtypes. We then examined the correlation between perilipin 2 immunoreactivity and intracellular lipid droplet evaluated by Oil‐red O stating in 13 cases of breast carcinoma tissues. A significantly positive correlation was detected between the status of perilipin 2 and Oil‐red O staining. These findings above did indicate that perilipin 2 could represent the status of intracellular lipid droplets in surgical pathology specimens of breast cancer and perilipin 2 was also associated with its more aggressive biological phenotypes.