Premium
Immunohistochemistry using monoclonal antibody MsMab‐2 is useful to detect IDH1 R132L in intrahepatic cholangiocarcinoma
Author(s) -
Hayashi Akimasa,
Misumi Kento,
Shibahara Junji,
Kokudo Norihiro,
Kato Yukinari,
Fukayama Masashi
Publication year - 2016
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12459
Subject(s) - immunohistochemistry , intrahepatic cholangiocarcinoma , monoclonal antibody , pathology , idh1 , antibody , medicine , biology , immunology , gene , biochemistry , mutation
Immunohistochemical analysis using specific antibodies is a useful and convenient method to detect proteins altered by somatic mutations. We previously generated the rat monoclonal antibody MsMab‐2, which recognizes isocitrate dehydrogenase (IDH)1 R132L and IDH2 R172M. In the present study, we used an immunohistochemical method to examine MsMab‐2 immunoreactivity in 95 cases of intrahepatic cholangiocarcinoma, including five IDH1 R132L and one IDH2 R172M mutant cases confirmed by direct sequencing. Tissue microarray section slides of all IDH1/2‐mutant and wild‐type cases, as well as whole section slides of IDH1 R132L and IDH2 R172M cases were immunostained using an autostainer. All IDH1 R132L cases showed positive staining for MsMab‐2, while other IDH1/2 mutant and IDH1/2 wild‐type cases were negative. Tumor cells of the immunopositive cases invariably showed strong reactivity using whole‐section slides. We consider immunohistochemical analysis using MsMab‐2 to be a useful means of detecting IDH1 R132L. Further analysis of its effectiveness against IDH2 R172M is necessary because of the small sample size in this study.