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Phosphohistone‐ H 3 ( PHH3 ) is prognostic relevant in M erkel cell carcinomas but M erkel cell polyomavirus is a more powerful prognostic factor than AJCC clinical stage, PHH3 , K i‐67 or mitotic indices
Author(s) -
Iwasaki Takeshi,
Matsushita Michiko,
aka Daisuke,
Kato Masako,
Nagata Keiko,
Murakami Ichiro,
Hayashi Kazuhiko
Publication year - 2015
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12305
Subject(s) - mitotic index , h&e stain , immunohistochemistry , mitosis , cell , medicine , pathology , biology , microbiology and biotechnology , genetics
M erkel cell carcinomas ( MCCs ) associated with M erkel cell polyomavirus ( MCPyV ) have better prognosis than those without MCPyV . The relationship between mitotic index ( MI ) and MCC outcome has remained elusive because of the difficulty in differentiating mitotic cells from apoptotic ones. We evaluated the role of phosphohistone‐ H 3 ( PHH3 ) ( S er10), a new mitotic count biomarker, in MCPyV ‐positive or ‐negative MCC patients, and assessed its prognostic value in comparison to Ki‐67 labeling index or MI using hematoxylin and eosin ( HE ) staining. We compared the prognostic value of PHH3 mitotic index with that of MI by HE in 19 MCPyV ‐positive and 9 MCPyV ‐negative MCC patients. PHH3 ‐positive immunoreactivity was mostly observed in mitotic figures. Multivariate analysis significantly showed that MCPyV status ( HR , 0.004; 95% CI 0.0003–0.058) and the A merican J oint C ommittee of C ancer ( AJCC ) stage ( HR , 5.02; 95% CI 1.23–20.51) were observed as significantly independent prognostic factors for OS . PHH3 ‐positive cell counts/10 HPF was a slightly significant independent prognostic factor for OS ( HR , 4.96; 95% CI 0.93–26.55). PHH3 ‐positive MI and MCPyV status in MCC patients are useful in prognostication, although MCPyV ‐infection is a more powerful prognostic factor in MCCs than the AJCC scheme on proliferation or mitotic indices.