Premium
Role of blood ribosomal protein S 19 in coagulum resorption: A study using G ln137 G lu‐ribosomal protein S 19 gene knock‐in mouse
Author(s) -
Chen Jun,
Fujino Rika,
Zhao Rui,
Semba Umeko,
Araki Kimi,
Yamamoto Tetsuro
Publication year - 2014
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12208
Subject(s) - ribosomal protein , microbiology and biotechnology , ribosomal rna , chemistry , resorption , gene , biology , biochemistry , rna , ribosome , endocrinology
Sera of human, guinea pig or mouse contain a strong monocyte chemoattractant capacity that is attributed to the ribosomal protein S 19 ( RP S19 ) oligomers generated during blood coagulation. In contrast, sera prepared from G ln137 G lu‐ RP S19 gene knock‐in mice contained negligible chemoattractant capacity. When coagula that had been pre‐formed from the blood of both the wild type and knock‐in mice were intraperitoneally inserted into host mice, after 3 days of recovery, the knock‐in mouse coagula remained larger than the wild type mouse coagula. The wild type mouse coagula were covered by multiple macrophage layers at the surface and were infiltrated inside by macrophages. Knock‐in mouse coagula exhibited less macrophage involvement. When coagula of knock‐in mice and coagula of knock‐in mice containing C 5a/ RP S19 , an artificial substitute of the RP S19 oligomers, were intraperitoneally inserted as pairs, the C 5a/ RP S19 containing coagulum was more rapidly absorbed, concomitant with increased macrophage involvement. Finally, when the knock‐in mouse and wild type mouse coagula pairs were inserted into mice in which macrophages had been depleted using clodronate liposome, the size difference of recovered coagula was reversed. These results indicate the importance of the RP S19 oligomer‐induced macrophage recruitment in coagulum resorption.