Premium
Immunohistochemical staining patterns of cytokeratins 13, 14, and 17 in oral epithelial dysplasia including orthokeratotic dysplasia
Author(s) -
Nobusawa Aiko,
Sano Takaaki,
Negishi Akihide,
Yokoo Satoshi,
Oyama Tetsunari
Publication year - 2014
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12125
Subject(s) - pathology , immunohistochemistry , dysplasia , staining , medicine
Diagnosis of the exact grade of oral epithelial dysplasia is difficult, and interobserver variations in grading are common. The aim of this study was to investigate the expression patterns of cytokeratins ( CKs ) in dysplastic oral epithelia, to identify useful double immunostaining diagnostic markers. Immunoexpression of CK 13, CK 14, CK 17, and K i‐67 were investigated in 21 normal epithelial specimens and 146 epithelial dysplasia specimens. In epithelial dysplasia specimens, orthokeratotic dysplasia ( OKD ) was identified using CK 10 immunostaining. Most mild dysplasia specimens were CK 13+ and CK 17–. In moderate dysplasia, CK 13 expression tended to be lower and CK 17 expression tended to be higher than in mild dysplasia. All carcinoma in situ ( CIS ) specimens were CK 17+. In differentiated type CIS specimens, CK 13 expression was weakly positive. Most epithelial dysplasia specimens were CK 14+. There were no significant differences in the expression patterns of CKs between OKD and non‐ OKD specimens in any of the grades of dysplasia. These results indicate that CK 14 expression can be used to detect early epithelial dysplasia, and that CK 13 and CK 17 expression are useful for detecting neoplastic changes.