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Fatty acid binding protein 7 as a marker of glioma stem cells
Author(s) -
Morihiro Yusuke,
Yasumoto Yuki,
Vaidyan Linda Koshy,
Sadahiro Hirokazu,
Uchida Tomoyuki,
Inamura Akinori,
Sharifi Kazem,
Ideguchi Makoto,
Nomura Sadahiro,
Tokuda Nobuko,
Kashiwabara Shoji,
Ishii Aya,
Ikeda Eiji,
Owada Yuji,
Suzuki Michiyasu
Publication year - 2013
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12109
Subject(s) - glioma , astrocytoma , anaplastic astrocytoma , immunohistochemistry , immunostaining , pathology , fatty acid binding protein , stem cell , immunocytochemistry , cancer research , biology , neural stem cell , medicine , biochemistry , microbiology and biotechnology , gene
Glioblastomas are the most aggressive brain tumors. Glioblastoma stem cells ( GSCs ) are thought to be responsible for the recurrence, chemoresistance, and poor prognosis of glioblastoma. Fatty acid binding protein 7 ( FABP 7), which is a cellular chaperone for a variety of omega‐3 fatty acids, is a known marker for neural stem cells. In this study, using a newly developed anti‐ FABP 7 antibody and patient‐derived GSC lines, we evaluated the expression of FABP 7 in GSCs . Using immunocytochemistry, W estern blotting, and qPCR analyses, FABP 7 was found to be highly enriched in GSCs and its localization was found in cytosol and nuclei. FABP 7 expression was significantly downregulated in differentiated GSCs induced by the addition of serum. In the glioma surgical specimens, FABP 7 was highly expressed in the majority of glioblastoma. Double immunostaining for FABP 7 and S ox2 showed that FABP 7 + S ox2 + tumor cells were significantly increased in glioblastoma (grade IV ) compared with diffuse astrocytoma (grade II ) and anaplastic astrocytoma (grade III ). Our data introduces FABP 7 as a marker for GSCs and further highlights its possible significance for glioma diagnosis and treatment.