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An immunohistochemical study of human platelets using a rabbit antibody against H 18‐ K 24 of apolipoprotein CIII ( HATKTAK )
Author(s) -
Yamanaka Takao,
Sakamoto Haruhiko,
Nakagawa Toshitaka,
Tanaka Sumiko,
Matsumoto Kouichi,
Ueno Masaki
Publication year - 2013
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12083
Subject(s) - platelet , antibody , chemistry , immunohistochemistry , immunoelectron microscopy , microbiology and biotechnology , fibrin , incubation , pathology , immunology , biology , biochemistry , medicine
H 18‐ K 24 of human apolipopotein CIII ( A po CIII ) ( HATKTAK ) is an activator of the macromolecular activators of phagocytosis from platelets ( MAPPs ). Using a rabbit antibody against HATKTAK , we performed an immunohistochemical study of human platelets. Indirect ELISA showed that this antibody reacts with A po CIII ‐derived peptides with a C ‐terminal of HATKTAK , but not with A po CIII . Immunoelectron microscopy revealed that reaction of anti‐ HATKTAK antibody occurred in the pseudopods of activated platelets. In blood coagula produced from the peripheral blood and formalin‐fixed after various incubation periods, reaction of this antibody with platelets appeared rapidly with a peak at 3 to 6 h of incubation, and then diminished gradually. Leukocytes in the blood coagula were stained strongly positive. In tissue sections, fresh thrombi and hemorrhages with slight fibrin formation revealed a positive response of platelets to anti‐ HATKTAK antibody, whereas older ones with leukocytic infiltration, fibrin formation and organization did not. In addition to platelets, endothelial cells and leukocytes were stained positive by anti‐ HATKTAK antibody. All of the positive reactions by anti‐ HATKTAK antibody disappeared or diminished by co‐incubation with HATKTAK . In conclusion, the anti‐ HATKTAK antibody reveals platelets during the early phase of activation.