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Expression of ECRG 4 is associated with lower proliferative potential of esophageal cancer cells
Author(s) -
Matsuzaki Junichi,
Torigoe Toshihiko,
Hirohashi Yoshihiko,
Tamura Yasuaki,
Asanuma Hiroko,
Nakazawa Emiri,
Saka Eri,
Yasuda Kazuyo,
Takahashi Shuji,
Sato Noriyuki
Publication year - 2013
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12079
Subject(s) - immunohistochemistry , cell growth , cancer research , biology , apoptosis , cell , immunology , genetics , biochemistry
We have shown that ECRG 4 suppressed F as‐induced apoptosis in J urkat cells. ECRG 4 mRNA expression was ubiquitously detected in normal adult human tissues, suggesting that ECRG 4 plays a major role in human tissues. ECRG 4 mRNA expression was down‐regulated in tumor cells. Expression of ECRG 4 suppressed cell growth. We established an anti‐ ECRG 4 monoclonal antibody. Our immunohistochemical analysis demonstrated that ECRG 4‐positive cells tended to be distributed in the region that was negative for K i‐67 in esophageal squamous cell carcinoma tissues. There was a significant inverse correlation between ECRG 4 expression and K i‐67 labeling index in esophageal squamous cell carcinoma. This study provides the first functional evidence for an association of endogenous expression of ECRG 4 with cell proliferation. ECRG 4 is a candidate tumor suppressor gene that might be involved in the proliferation of esophageal squamous cell carcinoma.