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Validity and reproducibility of K i‐67 assessment in gastrointestinal stromal tumors and leiomyosarcomas
Author(s) -
Ogino Jiro,
Asanuma Hiroko,
Hatanaka Yutaka,
Matsuno Yoshihiro,
Gotoda Hiroko,
Muraoka Shunji,
Tsuji Takayuki,
Fukazawa Yuichiro,
Yamashiro Katsushige,
Kondo Nobuo,
Iwaki Hiroyuki,
Miyokawa Naoyuki,
Hasegawa Tadashi
Publication year - 2013
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12038
Subject(s) - concordance , tissue microarray , immunohistochemistry , pathology , stromal cell , ki 67 , medicine , immunostaining , gastrointestinal tract , reproducibility , leiomyosarcoma , chemistry , chromatography
With the aim of standardizing K i‐67 immunohistochemistry, we assessed interobserver and interlaboratory variability of the K i‐67 labeling index and K i‐67 score among eight general pathologists for 24 gastrointestinal stromal tumors ( GISTs ) and 12 leiomyosarcomas, which were predominantly of the gastrointestinal ( GI ) tract, mesentery and retroperitoneum, based on a review of a tissue microarrays subjected to immunohistochemistry with antibodies for K i‐67. For K i‐67 immunostaining of mesenchymal tumors of the GI tract, including GISTs , differences were seen in the scores given by regional hospitals. Conversely, for two categories of the K i‐67 labeling index, namely <10% and ≥10%, concordance of the K i‐67 score between microscopic observation and image analysis, and between the observers, was good, but it was not good for the other four categories of the index for <5%, 5–9%, 10–29%, and ≥30%. The concordance of the K i‐67 scores between the observers in two categories was higher using the K i‐67 pre‐stained tissue microarrays ( TMAs) within each participating institute than that using the K i‐67 stained TMAs between the participating institutes. The reproducibility of a 10% cut‐off value for the K i‐67 labeling index to predict the prognosis of GISTs was relatively high, but there is an urgent need to standardize the staining technique.