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Detection of HEY1‐NCOA 2 fusion by fluorescence in‐situ hybridization in formalin‐fixed paraffin‐embedded tissues as a possible diagnostic tool for mesenchymal chondrosarcoma
Author(s) -
Nakayama Robert,
Miura Yasuhiro,
Ogino Jiro,
Susa Michiro,
Watanabe Itsuo,
Horiuchi Keisuke,
Anazawa Ukei,
Toyama Yoshiaki,
Morioka Hideo,
Mukai Makio,
Hasegawa Tadashi
Publication year - 2012
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12022
Subject(s) - fluorescence in situ hybridization , chondrosarcoma , fish <actinopterygii> , fusion , chemistry , in situ , pathology , medicine , biology , gene , biochemistry , linguistics , philosophy , fishery , chromosome , organic chemistry
Mesenchymal chondrosarcoma ( MC ) is an extremely rare subtype of chondrosarcoma. A tumor specific fusion gene, HEY1‐NCOA 2 fusion, was recently identified in this tumor. The finding raises the possibility that the diagnosis of MC can be improved by examining the fusion gene. In the present study, we aimed to evaluate the efficacy of fluorescence in situ hybridization ( FISH ) in detecting HEY1‐NCOA 2 fusion for the diagnosis of MC . Specimens from 10 patients diagnosed with MC were used for the study. Dual‐color FISH was performed using two different probes that specifically hybridize to HEY 1 and NCOA 2 , respectively. Fusion signals were identified in all but two specimens, in which no signal was detected, presumably because of inadequate sample preparation. In accordance with results of a previous study, FISH analysis was highly sensitive in detecting HEY1‐NCOA 2 fusion in adequately prepared MC samples. The current study adds further support for the use of HEY1‐NCOA 2 fusion as a valid diagnostic marker for MC .