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Characterization of murine extracellular vesicles and Toxoplasma gondii infection
Author(s) -
Maia Marta Marques,
Cruz Allecineia Bispo,
Pereira Ingrid de Siqueira,
Taniwaki Noemi Nosomi,
Namiyama Gislene Mitsue,
PereiraChioccola Vera Lucia
Publication year - 2021
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12869
Subject(s) - toxoplasma gondii , microvesicles , biology , cd63 , toxoplasmosis , tetraspanin , extracellular vesicles , immune system , splenocyte , extracellular , immunology , titer , microbiology and biotechnology , virology , microrna , antibody , biochemistry , gene , cell
This study characterized extracellular vesicles (EVs) of sera from mice infected with Toxoplasma gondii or immunized with EVs derived T gondii . EVs were purified of sera from four groups (5 A/Sn mice/group). EV‐IM: Mice immunized with T gondii ‐released EVs; ACT: mice in acute infection; CHR: mice in chronic infection; and NI: normal mice. EVs were purified by ultracentrifugation. Concentration of serum‐derived EVs from NI group was smaller than EV‐IM, ACT and CHR groups. Most of the EVs from ACT and CHR groups were microvesicles, and they were bigger than the NI group. The same results were shown by Transmission Electron Microscopy. The presence of exosomes was shown in immunoblotting by tetraspanin (CD63 and CD9) evidence. Splenocytes of EV‐IM, CHR and NI groups were stimulated with T. gondii derived EVs. EV‐IM and CHR groups up‐expressed IFN‐γ; TNF‐α and IL‐17, when compared with the NI group. IL‐10 was up‐expressed only in the EV‐IM group. EV‐IM, ACT and CHR groups expressed more miR‐155‐5p, miR‐29c‐3p and miR‐125b‐5p than the NI group. Host‐ T gondii interaction can occur, also, via EVs. miRNAs participate in the modulation of cellular immune response against T gondii . These data give subsidies to propose the differentiation between infect or noninfect hosts by concentration of EVs.