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Immunoproteomic analysis of fish ectoparasite, Argulus siamensis antigens
Author(s) -
Das Priyanka,
Badhe Mohan R.,
Sahoo Pramoda Kumar,
Reddy Raudu Rajendra Kumar,
Suryawanshi Amol R.,
Mohanty Jyotirmaya
Publication year - 2021
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12837
Subject(s) - biology , antigen , proteome , silver stain , microbiology and biotechnology , biochemistry , immunology
Aim An immunoproteomic approach was followed to identify immunoreactive antigens of fish ectoparasite, Argulus siamensis with rohu ( Labeo rohita ) immune sera for screening of potential vaccine candidates. Materials and results The whole adult Argulus antigen was run in 2D electrophoresis with IEF in 7 cm IPG strips of pH 4‐7 and SDS‐PAGE with 12% acrylamide concentration. Two parallel gels were run; one was stained with silver stain, and the other was Western blotted to nitrocellulose paper (NCP) and reacted with rohu anti‐ A siamensis sera. Fourteen protein spots corresponding to the spots developed in NCP were picked from the silver‐stained gel and subjected to mass spectrometry in MALDI‐TOF/TOF. The MS/MS spectra were analysed in MASCOT software with taxonomy ‘other metazoa’ and the proteins identified based on similarity with the proteins from heterologous species. The gene ontology analysis revealed a majority of proteins being involved in binding activity in ‘molecular function’ and belonging to metabolic processes in ‘biologic process’ categories. The possibility of these proteins as vaccine candidates against A siamensis is discussed in the paper. Conclusion Three of the identified proteins namely, bromodomain‐containing protein, anaphase‐promoting complex subunit 5 and elongation factor‐2 could possibly serve as vaccine candidates against argulosis in carps.