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Protein deficiency during Trichinella spiralis infection impairs lung immunity against newborn larvae
Author(s) -
Vila Cecilia C.,
Saracino María P.,
Lombardo Tomás,
Falduto Guido H.,
Díaz Mariángeles,
Calcagno Marcela A.,
Pallaro Anabel N.,
Baldi Pablo C.
Publication year - 2021
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12820
Subject(s) - antibody dependent cell mediated cytotoxicity , trichinella spiralis , biology , antibody , immunology , weaning , immunity , immune system , antigen , endocrinology , monoclonal antibody
The goal of this study was to analyse the effects of a protein‐deficient (PD) diet on antibody‐dependent cell‐mediated cytotoxicity (ADCC) in vitro against newborn larvae (NBL) of Trichinella spiralis in the lungs of infected rats. Two groups of weaning Wistar rats received a PD diet (6.5% casein) and other two received a control diet (C, 20% casein). After ten days, one group of each diet was infected (PD I and C I ) with muscle larvae. Lung tissue extracts (LTE) and lung cell suspension (LCS) were obtained. PD I had lower titres of anti‐NBL antibodies in LTE than C I . In ADCC assays using control cells, NBL mortality percentage was lower with LTE from PD I than LTE from C I ( P < .01). In assays using control cytotoxic sera, ADCC was exerted by LCS from C I at all days post‐infection (p.i.), but only by LCS from 13 days p.i. from PD I . ADCC assays combining LTE and LCS from the same group showed a lower response for PD I than for C I ( P < .0001). LCS from PD I contained lower numbers of neutrophils, eosinophils and FcεRI + cells than C I . PD may diminish ADCC activity against T spiralis NBL in lungs through alterations in specific antibodies and effector cells.