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Trichomonas vaginalis ‐secreted cysteinyl leukotrienes promote migration, degranulation and MCP‐1 production in mast cells
Author(s) -
Lee Young Ah,
Nam Young Hee,
Min Arim,
Shin Myeong Heon
Publication year - 2020
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12789
Subject(s) - degranulation , prostaglandin d2 , mast cell , biology , secretion , chemotaxis , leukotriene c4 , trichomonas vaginalis , leukotriene , leukotriene b4 , cytokine , receptor , inflammation , microbiology and biotechnology , immunology , endocrinology , biochemistry , asthma
Trichomonas vaginalis , a flagellated extracellular protozoan parasite that infects the human genitourinary tract, is usually transmitted by sexual contact. Our previous study showed that the leukotriene B 4 (LTB 4 ), a T vaginalis‐ secreted lipid mediator, induces interleukin (IL)‐8 production and promotes mast cell degranulation and migration via BLT1 in human. In this study, we investigated whether T vaginalis produces another leukotrienes and whether it causes increased MCP‐1 production, mast cell migration and degranulation by activating mast cells. We found that cysteinyl leukotrienes (CysLTs) were contained in T vaginalis ‐derived secretory product (TvSP) by ELISA. The TvSP‐stimulated human mast cell line (HMC‐1) exhibited significantly increased monocyte chemoattractant protein‐1 (MCP‐1) secretion compared to the unstimulated cells. Inhibition of NOX2 activation of cells by treatment of NOX inhibitor or NOX2 siRNA reduced TvSP‐stimulated MCP‐1 production in HMC‐1 cells. It was also confirmed that the receptor for CysLTs is expressed in mast cells. The CysLT receptor (CysLTR) antagonist inhibited TvSP‐stimulated MCP‐1 production of mast cells, as well as ROS production, migration and degranulation of mast cells, and reduced phospho‐NF‐kB expression. These results suggest that T vaginalis ‐secreted CysLTs promote migration, degranulation and MCP‐1 production in human mast cells through CysLT receptor‐mediated NOX2 activation.