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Association of cytokine gene polymorphisms with susceptibility to cutaneous leishmaniasis in a Turkish population
Author(s) -
Kirik Fatma Esin,
Ülger Mahmut,
Tezcan Ülger Seda,
Aslan Gönül
Publication year - 2020
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12775
Subject(s) - genotype , allele , biology , allele frequency , polymerase chain reaction , immunology , polymorphism (computer science) , turkish population , gastroenterology , medicine , genetics , gene
Aims The objective of this study was to determine the association of TNF‐α −308 G/A, IFN‐γ +874 T/A, IL‐12B + 1188 A/C, IL‐10 −1082 G/A and IL‐4 −590 C/T polymorphisms with susceptibility to CL. Methods and Results A total of 55 CL patients and 110 controls from Sanlıurfa province of Turkey were included to this study. Polymorphisms were genotyped by ‘polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP)’ and ‘amplification refractory mutation system‐PCR (ARMS‐PCR)’ methods. A statistically significant difference was noted in the allele ( P < .001, P = .002) and genotype ( P < .001, P = .001,) frequencies of TNF‐α −308 G/A and IL‐4 −590 C/T, respectively. TNF‐α 308 GG versus GA genotype (OR = 19.556 [95% CI 8.310‐46.019] P < .001), GG versus GA + AA genotype (OR = 20.444 [95% CI 8.707‐48.004] P < .001) and G versus A allele (OR = 6.968 [95% CI 3.903‐12.440] P < .001) revealed significant association with CL. IL‐4 −590 CC versus TT + CT genotype (OR = 2.049 [95% CI 1.025‐4.096], P = .041) and C versus T allele (OR = 2.441 [95% CI 1.355‐4.396], P = .002) revealed significant association with CL. Conclusion Our study indicates that TNF‐α 308 G/A and IL‐4‐590 C/T polymorphisms are significantly associated with susceptibility to CL. Individuals carrying A allele at TNF‐α promoter −308 position and T allele at IL‐4 promoter −590 position are at a higher risk for CL.