Premium
Toxoplasma gondii virus‐like particle vaccination alleviates inflammatory response in the brain upon T gondii infection
Author(s) -
Kang HaeJi,
Chu KiBack,
Lee SuHwa,
Kim MinJu,
Park Hyunwoo,
Jin Hui,
Moon EunKyung,
Quan FuShi
Publication year - 2020
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12716
Subject(s) - toxoplasma gondii , immunology , vaccination , biology , rhoptry , antibody , neuroinflammation , virology , immune system , cytokine , toxoplasmosis , inflammation , malaria , apicomplexa , plasmodium falciparum
Aims Neuroinflammation can manifest upon infection with the neurotropic parasite Toxoplasma gondii (ME49), which can lead to brain injury and cognitive dysfunction. Rhoptry organelle proteins (ROPs) secreted by T gondii play critical roles in host invasion. Methods and results In this study, influenza virus‐like particles (VLPs) expressing T gondii ROP4 or ROP13 were generated to assess vaccination‐induced changes in intracranial pro‐inflammatory cytokines and antibody responses upon T gondii challenge infection. Compared to ROP13 VLPs, ROP4VLPs vaccination significantly limited the production of pro‐inflammatory cytokines IFN‐γ and IL‐6 in the brains of mice. Reduced pro‐inflammatory cytokine responses by ROP4 VLPs and ROP13 VLPs correlated with significantly increased T gondii ‐specific IgG and IgA antibody responses in the brain, as well as IgG, IgG1 and IgM antibody responses in the sera. Conclusion We concluded that influenza T gondii VLP vaccination induces antibody responses in sera and brain, which may contribute to the significant reduction of neuroinflammation during T gondii infection.