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Neutrophil extracellular traps and MPO in models of susceptibility and resistance against Entamoeba histolytica
Author(s) -
Contis Montes de Oca Arturo,
Cruz Baquero Andrea,
Campos Rodríguez Rafael,
Cárdenas Jaramillo Luz María,
Aguayo Flores José Eduardo,
Rojas Hernández Saúl,
Olivos García Alfonso,
Pacheco Yepez Judith
Publication year - 2020
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12714
Subject(s) - myeloperoxidase , neutrophil extracellular traps , entamoeba histolytica , hamster , biology , elastase , effector , neutrophil elastase , extracellular , in vitro , immunology , microbiology and biotechnology , mesocricetus , inflammation , enzyme , biochemistry
The main effector mechanisms of neutrophils are the release of neutrophil extracellular traps (NETs) and myeloperoxidase (MPO). In this work, we evaluated the role of NETs and the activity of MPO in the interactions of rodent neutrophils with amoebae and in amoebic liver abscess (ALA)‐resistant and ALA‐susceptible models. We showed with in vitro assays that mice produced greater amounts of NETs and MPO than did hamsters, and the elastase activity was high in both models. However, the inhibition of NETs and MPO promoted an increase in amoeba viability in the mice. The mouse ALAs showed a more profound presence of NETs and MPO than did the hamster ALAs. We concluded that both effector mechanisms were essential for the amoebic damage and could prevent the formation of ALAs in the resistant model.