Premium
Neutrophils rapidly produce Th2 cytokines in response to larval but not adult helminth antigen
Author(s) -
Middleton Denzel,
Garza Javier J.,
Greiner Scott P.,
Bowdridge Scott A.
Publication year - 2020
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12679
Subject(s) - biology , immunology , helminths , antigen , larva , helminth infections , helminthiasis , immune system , ecology
Host protective immunity to Haemonchus contortus (Hc) infection in parasite‐resistant St. Croix (STC) sheep is initiated early and characterized by an influx of innate cells and robust interleukin‐4 (IL‐4) production, resulting in T‐helper type 2 immune (Th2) responses. The purpose of these studies was to elucidate the source of early IL‐4 production. Neutrophils were isolated from whole blood, and populations >98% purity were cultured with larval or adult antigen to access cytokine production. Interleukin‐4 and IL‐13 were measured in sample supernatant using an ovine‐specific enzyme‐linked immunosorbent assay (ELISA). Neutrophils exposed to HcLA peaked in IL‐4 production at 30 minutes (STC, 3153.65 pg/mL and SUF, 4665.22 pg/mL). A similar trend was observed in IL‐13 production by 6 hours (STC, 391.02 pg/mL and SUF, 419.6 pg/mL). Adult antigen stimulation resulted in low cytokine production when compared to HcLA stimulation (STC IL‐4, 6.04 pg/mL and SUF, 8.05 pg/mL, respectively; STC IL‐13, 10 pg/mL and 12.5 pg/mL; P < .001), and no breed differences were observed. Mixed immune cell assays revealed an ability of neutrophils to induce IL‐4 production in peripheral blood mononuclear cell (PBMC). Taken together, these data implicate neutrophils as a potential effector cell responsible for Th2 initiation.