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A molecular signature for CD8 + T cells from visceral leishmaniasis patients
Author(s) -
Singh Bhawana,
Bhushan Chauhan Shashi,
Kumar Rajiv,
Singh Siddharth Sankar,
Ng Susanna,
Amante Fiona,
Labastida Rivera Fabian,
Singh Om Prakash,
Rai Madhukar,
Nylen Susanne,
Sundar Shyam,
Engwerda Christian
Publication year - 2019
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12669
Subject(s) - biology , immunology , cd8 , perforin , cytotoxic t cell , granzyme b , granzyme , immune system , population , t cell , medicine , genetics , environmental health , in vitro
CD8 + T‐cell function is compromised in chronic diseases such as visceral leishmaniasis (VL). However, little is known about the changes in gene expression that cause CD8+ T‐cell dysfunction during VL. We used targeted transcriptional profiling of peripheral blood CD8 + T cells from VL patients pre‐ and post‐anti‐parasitic drug treatment, and compared them with the same cell population from healthy endemic controls to assess their activation, differentiation and functional status during disease. We found a predominance of downregulated immune genes in CD8 + T cells from VL patients. However, genes encoding several notable immune checkpoint molecules, including LAG‐3, TIM‐3 and CTLA‐4, cytolytic molecules, such as granzymes A, B and H and perforin, as well as SOCS3, STAT1, JAK2 and JAK3 cytokine signalling genes were found to be increasingly expressed by VL patient CD8 + T cells. Additional studies confirmed increased expression of the inhibitory receptors LAG3 and TIM3 on VL patient CD8 + T cells, thereby identifying these molecules as potential targets to improve antigen‐specific CD8 + T‐cell responses during disease.