Premium
Administration of anti‐CTLA‐4 monoclonal antibody augments protective immunity induced by Schistosoma japonicum glutathione‐S‐transferase
Author(s) -
Tang Chunlian,
Pan Qun,
Dai Wenqin,
Hu Lidong,
Zhou Honghua,
Wang Lixia
Publication year - 2019
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12657
Subject(s) - schistosoma japonicum , monoclonal antibody , biology , ctla 4 , immunology , immune system , glutathione s transferase , immunization , adjuvant , antibody , immunity , virology , glutathione , t cell , schistosomiasis , enzyme , helminths , biochemistry
Aims The aim of this study was to evaluate the effect of anti‐CTLA‐4 monoclonal antibody (mAb) on 26‐kDa glutathione‐S‐transferase (GST) vaccine–induced immunity against Schistosoma japonicum infection. Methods and Results Mice immunized with GST before infection with S japonicum cercariae were injected with anti‐CTLA‐4 mAb. Worm reduction rate of GST was increased from 25.41% in mice with GST immunization to 52.48% in mice with GST plus anti‐CTLA‐4 mAb. The percentages of regulatory T cells (Tregs) were significantly higher following administration of both GST and anti‐CTLA‐4 mAb, or anti‐CTLA‐4 mAb alone. Elevated levels of IFN‐γ, IL‐2, IL‐4 and IL‐5 were observed. Conclusion These results demonstrated that CTLA‐4 may inhibit the protective effect of GST vaccine, and anti‐CTLA‐4 mAb may be used as an adjuvant to enhance the immune protection conferred by the GST vaccine by enhancing Th1‐ and Th2‐type immune response.