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Y75B8A.8 ( HC8 ) protein of Haemonchus contortus : A functional inhibitor of host IL‐2
Author(s) -
Wang Wenjuan,
Wang Yujian,
Tian Xiaowei,
Lu Mingmin,
Ehsan Muhammad,
Yan RuoFeng,
Song XiaoKai,
Xu LiXin,
Li XiangRui
Publication year - 2019
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12625
Subject(s) - haemonchus contortus , biology , immune system , peripheral blood mononuclear cell , host (biology) , immunology , interleukin , cytokine , biochemistry , helminths , genetics , in vitro
Summary Interleukin 2 ( IL‐2 ) is an important immune regulatory factor in the immune response of the host. However, little is known about the inhibitor of host IL‐2 in Haemonchus contortus infection. In this study, we found that globin domain‐containing protein (HCGB ) and Protein Y75B8A.8 ( HC8 ) from H contortus excretory and secretory products are two binding proteins of IL‐2 in goats. The yeast two‐hybrid screening further validated the positive interactions of IL‐2 with HCGB and HC8 . Meanwhile, we found that HC8 had inhibitory effects on IL‐2 ‐induced peripheral blood mononuclear cell (PBMC) proliferation, while HCGB did not. Furthermore, transcriptional analysis revealed that HC8 could block the IL‐2 ‐activated signalling pathway. Our results showed that HC8 was a functional inhibitor of goat IL‐2 .