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Leishmania braziliensis isolated from disseminated leishmaniasis patients downmodulate neutrophil function
Author(s) -
Cardoso Thiago,
Bezerra Caroline,
Medina Lilian Silva,
Ramasawmy Rajendranath,
Scheriefer Albert,
Bacellar Olívia,
Carvalho Edgar M.
Publication year - 2019
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12620
Subject(s) - leishmania braziliensis , biology , respiratory burst , immunology , cutaneous leishmaniasis , leishmaniasis , leishmania , microbiology and biotechnology , parasite hosting , flow cytometry , amastigote , world wide web , computer science
Summary Aims The polymorphism observed in Leishmania braziliensis is associated with different clinical forms of leishmaniasis. Neutrophils ( PMN s) participate in the pathogenesis of leishmania infection, and here, we evaluate neutrophil function after infection with isolates of L. braziliensis from cutaneous leishmaniasis ( CL ) or disseminated leishmaniasis ( DL ) patients. Methods and results Neutrophils from 30 healthy subjects ( HS ) were infected with isolates of L . ( V .) braziliensis obtained from three CL and three DL patients. They were infected at the ratio of 3:1 parasites per neutrophil, and leishmania uptake was evaluated by microscopy. The neutrophil activation markers and oxidative burst by expression of dihidrorhodamine ( DHR ) were evaluated by flow cytometry and cytokine production by ELISA . The frequency of infected cells and the number of amastigotes were higher in neutrophils infected with CL isolates compared to DL isolates ( P < 0.05). The DHR and CD 66b expression after infection with DL isolate was lower than with CL isolates. There was no difference regarding chemokine production. Conclusion The L. (V.) braziliensis isolates of DL induced lower respiratory burst and neutrophils activation markers compared with CL isolates which may contribute to parasite survival and dissemination in DL patients.