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Association of polymorphisms of the β‐defensin 1 gene with nematode and protozoan infection traits in goat
Author(s) -
Maia Flora Suzane Parente,
Campelo José Elivalto Guimarães,
Sarmento José Lindemberg Rocha,
Silva Caio Santos,
Marques José Ribamar Felipe,
Alves Francisco Arimatéia Santos,
Guimarães Rafaelle Casseb,
Filho Ednaldo Silva
Publication year - 2019
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12613
Subject(s) - biology , snp , single nucleotide polymorphism , genotype , genetics , exon , gene , genetic marker , genetic association , dna extraction , polymerase chain reaction
Summary This study's purpose was to identify polymorphisms ( SNP ) in the goat β‐defensin 1 gene and to associate these SNP s with traits related to nematodean and protozoan infections in Anglo‐Nubian goats from semiarid region of Brazil. A total of 184 animals were used for DNA extraction, PCR and DNA automatic sequencing. The association analyses included the fixed effects of animal age, bloodline and genotype of the SNP marker in the statistical model. The means of genotypes were compared by the Fisher test ( P < 0.05). Twelve polymorphism genotypes were found: two in intron 1, seven in exon 2 and three in 3′ untranslated region. The SNP s of exon 2 were responsible for amino acid substitutions in six genetic codes, and the changes in the 25th and 33rd codes affected the protein function. The SNP 1937 was significantly associated with number of protozoan oocysts, whereas SNP 2001 was associated with degree of anaemia (Famacha©). Polymorphism 2046, in turn, showed a significant association with Famacha© degree and number of protozoan oocysts. SNP 2140 associated with maximum EPG of the animal. Results from this study suggest that the β‐defensin 1 gene can be used as a molecular marker for selection of goats regarding the susceptibility to endoparasites infections.