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Inducible nitric oxide synthase controls experimental Strongyloides infection
Author(s) -
Rodrigues Rosângela Maria,
Gonçalves Ana Lúcia Ribeiro,
Silva Neide Maria,
Cardoso Cristina Ribeiro de Barros,
Araújo Natália Rodrigues,
Coutinho Loyane Bertagnolli,
Alves Ronaldo,
Ueta Marlene Tiduko,
CostaCruz Julia Maria
Publication year - 2018
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12576
Subject(s) - nitric oxide synthase , biology , nitric oxide , immune system , immunology , strongyloides , strongyloidiasis , nippostrongylus brasiliensis , immunoglobulin e , parasite hosting , enzyme , microbiology and biotechnology , antibody , helminths , endocrinology , biochemistry , world wide web , computer science
Summary Infection with Strongyloides sp. induces a host immune response, predominantly the Th2 type, that is able to eliminate the parasite. However, little is known about the role of the nitric oxide ( NO ) mediator, induced by the enzyme nitric oxide synthase ( NOS ), in strongyloidiasis. Therefore, in this study, we investigated the immune response of mice genetically deficient in the enzyme inducible nitric oxide synthase ( iNOS −/− ), infected with Strongyloides venezuelensis . C57 BL /6 wild‐type ( WT ) and iNOS −/− mice were individually inoculated by subcutaneous injection of 3000 S. venezuelensis L3 larvae. In the absence of iNOS , mice were more susceptible to the infection than WT animals, in which the parasite was completely eliminated. The overall production of cytokines and specific IgG, IgG1 or IgE antibodies against the parasite was significantly lowered in infected iNOS −/− mice. The expression of iNOS was observed in the intestine of WT hosts but mainly in the wall of the parasite, despite the presence of iNOS in mice. Altogether, we concluded that iNOS expression may play an important role in the control of S . venezuelensis infection.