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Reactivity of recombinant Toxocara canis TES‐30/120 in experimentally infected mice
Author(s) -
Santos Lucas Moreira dos,
Moura Micaele Quintana,
Azevedo Morgana Lüdtke,
Marques Giuli Argou,
Avila Luciana Farias da Costa,
Scaini Carlos James,
Berne Maria Elisabeth,
Moreira Ângela Nunes,
Conceição Fabricio Rochedo
Publication year - 2018
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12568
Subject(s) - toxocara canis , biology , recombinant dna , immunology , toxocariasis , canis , virology , helminths , ecology , biochemistry , gene
Aim While the use of recombinant antigens is being widely investigated in the diagnosis of human toxocariasis, relatively little attention has been given to animal diagnostic models. For this reason, this study aimed to investigate the diagnosis potential of Toxocara canis TES‐30 and TES‐120 recombinant antigens in mice, the animal model for toxocariasis studies. Methods and results Serum samples obtained from mice infected with T. canis or Toxocara cati were tested by indirect ELISA using T. canis TES‐30 and TES‐120 recombinant antigens produced in Escherichia coli . 90% of the samples reacted with rTES‐30, whereas there was almost no reactivity with rTES‐120. Conclusion Despite rTES‐120 being a good antigen for diagnosis in humans, it could not reproduce its reactivity in this animal model. As rTES‐30 has good reactivity in mice, it is a valuable tool for diagnosis.