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Eosinophilic inflammation in lymph nodes of dogs with visceral leishmaniasis
Author(s) -
Costa Sidnei Ferro,
Trivellato Gabriel Franco,
Rebech Gabriela Torres,
Oliveira dos Santos Maciel Marilene,
Melo Larissa Martins,
Luvizotto Maria Cecília Rui,
Lima Valéria Marçal Felix
Publication year - 2018
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12567
Subject(s) - visceral leishmaniasis , eosinophil , lymph , immunology , parasite load , leishmaniasis , biology , biopsy , leishmania , pathology , lymph node , immune system , medicine , parasite hosting , asthma , world wide web , computer science
Summary Eosinophils are traditionally associated with the immune response against helminth parasites. However, several studies have demonstrated that these cells have a role regarding protective immunity in leishmaniasis. Here, we examined the relationship between the presence of eosinophils and parasite load in biopsy samples from dogs, obtained through fine needle puncture and aspiration of lymph nodes. Histological slides containing biopsy material from lymph nodes of dogs with canine visceral leishmaniasis and healthy dogs were used to obtain baseline eosinophil counts. Subsequently, scrapings were taken from slides for DNA extraction and determination of parasite load, using real‐time PCR (qRT‐PCR). Additionally, production of nitric oxide (NO) and reactive oxygen species (ROS) levels by eosinophils in the peripheral blood of dogs with canine visceral leishmaniasis and healthy dogs was measured. The eosinophil percentage were higher in lymph nodes of infected group, and the parasite load showed a significant negative correlation with the eosinophil count. The production of NO and ROS by eosinophils in the peripheral blood was higher in the dogs with canine visceral leishmaniasis. All the results together suggest that eosinophils may participate in antileishmanial immunity in canine visceral leishmaniasis.