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Fcγ‐ RI , Fcγ‐ RII and IL ‐10 as predictive biomarkers for post‐therapeutic cicatrization time in monocytes from cutaneous leishmaniasis patients
Author(s) -
RodriguesAlves M. L.,
MeloJúnior O. A. O.,
CoelhodosReis J. G.,
PascoalXavier M. A.,
AlvesCosta H.,
Reis C. A.,
Dutra W. O.,
Silva R. E.,
Senna M. C. R.,
Faria A. C.,
Medeiros N. I.,
Gomes J. A. S.,
SilveiraLemos D.,
MartinsFilho O. A.,
TeixeiraCarvalho A.,
CostaSilva M. F.,
Giunchetti R. C.,
PeruhypeMagalhães V.
Publication year - 2018
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12565
Subject(s) - ex vivo , cutaneous leishmaniasis , flow cytometry , immunology , in vivo , leishmania braziliensis , biomarker , interleukin 10 , medicine , leishmaniasis , leishmania , interleukin , immune system , biology , cytokine , parasite hosting , world wide web , computer science , biochemistry , microbiology and biotechnology
Summary Cutaneous leishmaniasis ( CL ) treatment is based on therapy with Glucantime ® , yet, there are few laboratory methods to monitor its success. In this study, ex vivo and in vitro evaluations of peripheral blood monocytes were performed in a longitudinal study to characterize the impact of Glucantime ® on overall phenotypic/functional features of these cells from CL patients to identify predictive biomarkers for post‐therapeutic monitoring by flow cytometry. The ex vivo evaluation from CL patients demonstrated a modulatory profile before treatment, with a decrease in TLR ‐2, Fcγ RII , HLA ‐ DR , CD 86, IFN ‐γR, TNF , IL ‐12, NO , and an increase in Fcγ RIII and IL ‐10R. Conversely, treatment changes some of these biomarker expressions by decreasing Fcγ RIII and IL ‐10R and increasing IFN ‐γR, IL ‐12 and NO . Moreover, an in vitro analysis of these patients showed a reduced phagocytic capacity of Leishmania braziliensis and higher levels of IL ‐10 and TGF ‐β modulating functional profile. Regardless of the compromised L. braziliensis phagocytic capacity, treatment re‐established the production of IL ‐12, IL ‐10, TGF ‐β and NO at the basal level. Notably, monocytes from patients with early cicatrization showed enhanced Fcγ RI and Fcγ RII expressions and reduced IL ‐10, which was further corroborated by a baseline fold change analysis. Finally, the logistic regression model emphasized the performance of Fcγ RI , Fcγ RII and IL ‐10 as robust predictive biomarkers for post‐therapeutic cicatrization during cutaneous leishmaniasis.