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Anisakis pegreffii impacts differentiation and function of human dendritic cells
Author(s) -
Napoletano C.,
Mattiucci S.,
Colantoni A.,
Battisti F.,
Zizzari I. G.,
Rahimi H.,
Nuti M.,
Rughetti A.
Publication year - 2018
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12527
Subject(s) - biology , anisakis , chemokine , immune system , immunology , phenotype , monocyte , dendritic cell , microbiology and biotechnology , genetics , gene , larva , ecology
Summary Human dendritic cells ( DC s) show remarkable phenotypic changes when matured in the presence of helminth‐derived products. These modifications frequently elicited a polarization towards Th2 cells and regulatory T cells thus contributing to immunological tolerance against these pathogens. In this study, the interaction between DC s and larvae of the zoonotic anisakid nematode Anisakis pegreffii was investigated. A. pegreffii larvae were collected from fish hosts, and monocyte‐derived DC s were cocultured in the presence of the live larvae (L) or its crude extracts ( CE ). In both experimental conditions, A. pegreffii impacted DC viability, hampered DC maturation by reducing the expression of molecules involved in antigen presentation and migration (ie HLA ‐ DR , CD 86, CD 83 and CCR 7), increased the phagosomal radical oxygen species ( ROS ) levels and modulated the phosphorylation of ERK 1,2 pathway. These biological changes were accompanied by the impairment of DC s to activate a T‐cell‐mediated IFN γ. Interestingly, live larvae appeared to differently modulate DC secretion of cytokines and chemokines as compared to CE . These results demonstrate, for the first time, the immunomodulatory role of A. pegreffii on DC s biology and functions. In addition, they suggest a dynamic contribution of DC s to the induction and maintenance of the inflammatory response against A. pegreffii .