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Mannose receptor (MR) and Toll‐like receptor 2 (TLR2) influence phagosome maturation during Leishmania infection
Author(s) -
Polando R. E.,
Jones B. C.,
Ricardo C.,
Whitcomb J.,
Ballhorn W.,
McDowell M. A.
Publication year - 2018
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12521
Subject(s) - phagosome , mannose receptor , leishmania , biology , tlr2 , phagocytosis , phagolysosome , leishmania major , toll like receptor , immunology , lipophosphoglycan , microbiology and biotechnology , antibody opsonization , receptor , macrophage , leishmania donovani , innate immune system , immune system , leishmaniasis , opsonin , visceral leishmaniasis , in vitro , biochemistry , parasite hosting , world wide web , computer science
Summary Leishmania enter macrophages through receptor‐mediated phagocytosis and survive the harsh environment of a phagolysosome. Here, we investigated the interaction between mannose receptor (MR), Toll‐like receptor 2 (TLR2), and Leishmania , and the subsequent impact on phagosome maturation. Leishmania parasites are able to delay phagosome maturation, not reaching full maturation until 5 hours post‐engulfment. Here, maturation of Leishmania major‐ and Leishmania donovani‐ containing phagosomes proceeded as expected in the WT macrophages becoming LAMP 1 positive by 6 hours. Interestingly, MR −/− macrophages become LAMP 1 positive by ~2 hours and ~4 hours post‐infection Leishmania ‐containing phagosomes lost LAMP 1 expression and gained the early marker EEA 1. LAMP 1 expression was again observed by 6 hours. Leishmania LPG was essential for the delay in both WT and MR −/− macrophages but was not essential for the early maturation (2 hours) observed in MR −/− macrophages. Serum opsonization of Leishmania prior to infection induced identical phagosome maturation patterns in WT and MR −/− macrophages. In the absence of MyD88 or TLR 2 on macrophages, Leishmania phagosomes matured significantly faster, becoming LAMP 1 positive by ~1‐2 hours. These studies add to the knowledge that phagosome maturation is influenced by multiple receptor‐ligand interactions and signalling pathways