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Leishmania amazonensis induces modulation of costimulatory and surface marker molecules in human macrophages
Author(s) -
Costa S. S.,
Fornazim M. C.,
Nowill A. E.,
Giorgio S.
Publication year - 2018
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12519
Subject(s) - biology , internalization , downregulation and upregulation , immunology , immune system , mhc class i , major histocompatibility complex , microbiology and biotechnology , parasite hosting , leishmania , mhc class ii , receptor , gene , genetics , world wide web , computer science
Summary Manipulation of costimulatory and surface molecules that shape the extent of immune responses by Leishmania is suggested as one of the mechanisms of evading the host's defences. The experiments reported here were designed to evaluate the expressions of CD 11b, CD 11c, CD 14, CD 18, CD 54, CD 80, CD 86, CD 206, MHC class II and TLR ‐2 (Toll‐like receptor 2) in human macrophages infected with L. amazonensis . Phenotypic evaluation revealed a negative modulation in CD 11b, CD 11c, CD 14, CD 18, CD 54 and MHC class II molecules, depending on the level of infection. The results showed that as early as 1 hour after infection no reduction in marker expression occurs, whereas after 24 hours, downregulation of these molecules was observed in macrophages. No significant changes were observed in the expressions of CD 80, CD 86, CD 206 and TLR 2. Evidence of the differential modulation of markers expression and that after parasite uptake no reduction in surface marker expression occurs indicates that parasite internalization is not involved in the phenomena of down‐modulation.