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Local immune response to larvae of Rhipicephalus microplus in Santa Gertrudis cattle
Author(s) -
Constantinoiu C. C.,
LewTabor A.,
Jackson L. A.,
Jorgensen W. K.,
Piper E. K.,
Mayer D. G.,
Johnson L.,
Venus B.,
Jonsson N. N.
Publication year - 2018
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12515
Subject(s) - biology , tick , larva , rhipicephalus microplus , immune system , rhipicephalus , veterinary medicine , eosinophil , antigen , immunology , virology , ecology , medicine , asthma
Summary This study investigated the local immune response at larval attachment sites in Santa Gertrudis cattle with low and high levels of tick resistance. Skin samples with tick larvae attached were collected from Santa Gertrudis cattle at the end of a period of 25 weekly infestations, when the animals manifested highly divergent tick‐resistant phenotypes. There was a tendency for more CD 3 + , CD 4 + , CD 8 + , CD 25 + , γδ T cells and neutrophils to concentrate at larval tick attachment site in susceptible cattle than in resistant cattle but the differences were significant only for γδ T cells and CD 4 + cells. Most of the cattle developed intra‐epidermal vesicles at the larval attachment site but the predominant cell within or around the vesicles was the neutrophil in susceptible animals and eosinophil in the resistant animals. The monoclonal antibodies ( mA bs) specific for CD 45 and CD 45 RO antigens reacted with skin leucocytes from a higher number of susceptible cattle than resistant cattle. Our data suggest that some of the cellular responses mounted at larval attachment site are not involved in tick protection. The mA bs specific for CD 45 and CD 45 RO directly, or a test for CD 45 genotype might be developed as markers of tick susceptibility or resistance.