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Naegleria fowleri immunization modifies lymphocytes and APC of nasal mucosa
Author(s) -
CarrascoYepez M. M.,
CamposRodríguez R.,
ReséndizAlbor A. A.,
PeñaJuárez C.,
ContisMontes de Oca A.,
ArciniegaMartínez I. M.,
BonillaLemus P.,
RojasHernandez S.
Publication year - 2018
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12508
Subject(s) - biology , antibody , lymphatic system , nasal administration , spleen , immunology , flow cytometry , immune system , naegleria fowleri , antigen , mucous membrane of nose , immunization , cholera toxin , microbiology and biotechnology , meningoencephalitis
Summary We investigated whether intranasal immunization with amoebic lysates plus cholera toxin modified the populations of T and B lymphocytes, macrophages and dendritic cells by flow cytometry from nose‐associated lymphoid tissue ( NALT ), cervical lymph nodes ( CN ), nasal passages ( NP ) and spleen ( SP ). In all immunized groups, the percentage of CD 4 was higher than CD 8 cells. CD 45 was increased in B cells from mice immunized. We observed IgA antibody‐forming cell (IgA‐ AFC ) response, mainly in NALT and NP . Macrophages from NP and CN expressed the highest levels of CD 80 and CD 86 in N. fowleri lysates with either CT or CT alone immunized mice, whereas dendritic cells expressed high levels of CD 80 and CD 86 in all compartment from immunized mice. These were lower than those expressed by macrophages. Only in SP from CT ‐immunized mice, these costimulatory molecules were increased. These results suggest that N. fowleri and CT antigens are taking by APC s, and therefore, protective immunity depends on interactions between APC s and T cells from NP and CN . Consequently, CD 4 cells stimulate the differentiation from B lymphocytes to AFC IgA‐positive; antibody that we previously found interacting with trophozoites in the nasal lumen avoiding the N. fowleri attachment to nasal epithelium.