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Antischistosome antibodies change NTPD ase 1 activity from macrophages
Author(s) -
Marconato D. G.,
Gusmão M. A. do N.,
Melo J.,
Castro J. M. de A.,
Macedo G. C.,
Vasconcelos E. G.,
FariaPinto P.
Publication year - 2017
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12487
Subject(s) - biology , antibody , polyclonal antibodies , western blot , schistosoma mansoni , microbiology and biotechnology , enzyme , immune system , immunofluorescence , gene isoform , antigen , macrophage , blot , schistosomiasis , immunology , in vitro , biochemistry , helminths , gene
Summary NTPD ases are enzymes that hydrolyse diphosphate and triphosphate nucleosides, regulating purinergic signalling in many organisms. The Schistosoma mansoni NTPD ases , Sm ATPD ases 1 and 2, are antigenic proteins and display a significant homology with the isoforms found in mammalian cells. In this work, we investigated whether anti‐Sm ATPD ase antibodies from S. mansoni‐ infected mice sera show cross‐reactivity with the NTPD ase 1 isoform from macrophages and how this event affects the cell proliferation. By Western blot, anti‐Sm ATPD ase antibodies present in serum from infected mice recognized 2 bands with approximately 53 and 58 kD a, corresponding to NTPD ase 1. Additionally, the enzyme was identified in macrophages by immunofluorescence and the anti‐Sm ATPD ase antibodies were able to reduce activity enzyme (22%). Macrophages incubated with commercial polyclonal antibodies reactive with NTPD ase 1 (anti‐ CD 39) showed a reduction of 40% of the enzyme activity. In proliferation assays, macrophage proliferation was inhibited 11% and 90% by pooled sera from infected animals and anti‐ CD 39, respectively. The results suggest that inhibition of NTPD ase 1 in macrophages by antibodies produced against the isoforms of the S. mansoni ATPD ases could be a mechanism of regulation in the immune response during experimental schistosomiasis.