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Evaluation of gene expression levels for cytokines in ocular toxoplasmosis
Author(s) -
Maia M. M.,
MeiraStrejevitch C. S.,
PereiraChioccola V. L.,
Hippólito D. D. C.,
Silva V. O.,
Brandão de Mattos C. C.,
Frederico F. B.,
Siqueira R. C.,
Mattos L. C.
Publication year - 2017
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12462
Subject(s) - peripheral blood mononuclear cell , toxoplasmosis , toxoplasma gondii , immunology , biology , antigen , cytokine , messenger rna , in vitro , antibody , gene , biochemistry
Summary This study evaluated levels for mRNA expression of 7 cytokines in ocular toxoplasmosis. Peripheral blood mononuclear cells ( PBMC ) of patients with ocular toxoplasmosis ( OT Group, n = 23) and chronic toxoplasmosis individuals ( CHR Group, n = 9) were isolated and stimulated in vitro with T. gondii antigen. Negative controls ( NC ) were constituted of 7 PBMC samples from individuals seronegative for toxoplasmosis. mRNA expression for cytokines was determined by qPCR . Results showed a significant increase in mRNA levels from antigen stimulated PBMC s derived from OT Group for expressing IL ‐6 (at P < .005 and P < .0005 for CHR and NC groups, respectively), IL ‐10 (at P < .0005 and P < .005 for CHR and NC groups, respectively) and TGF ‐β (at P < .005) for NC group. mRNA levels for TNF ‐α and IL ‐12 were also upregulated in patients with OT compared to CHR and NC individuals, although without statistical significance. Additionally, mRNA levels for IL ‐27 and IFN ‐γ in PBMC of patients with OT were upregulated in comparison with NC individuals. Differences between OT and NC groups were statistically significant at P < .05 and P < .0005, respectively.