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Protective role of IL ‐22 against Schistosoma mansoni soluble egg antigen‐induced granuloma in Vitro
Author(s) -
Nady S.,
Shata M. T. M.,
Mohey M. A.,
ElShorbagy A.
Publication year - 2017
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12392
Subject(s) - schistosoma mansoni , granuloma , biology , immunology , granulocyte , antigen , in vitro , schistosomiasis , biochemistry , helminths
Summary The role of T helper‐17 (Th17) lymphocytes in the regulation of Schistosoma mansoni soluble egg antigen ( SEA )‐induced granuloma is unknown. This study examined the effect of Th17 cytokines ( IL ‐17 and IL ‐22) on granulocyte recruitment and functions during SEA ‐induced granuloma formation in vitro in Schistosoma‐ infected and noninfected individuals. Granulocytes were isolated from 27 Schistosoma‐infected patients and 13 controls and were used for granuloma induction using SEA ‐conjugated polyacrylamide beads in the presence of Th17 cytokines. Granuloma index was assessed, and granulocyte mediators such as tumour necrosis factor ( TNF ‐α), hydrogen peroxide (H 2 O 2 ) and nitric oxide ( NO ) were measured in the culture supernatant at the 7th day using enzyme‐linked immunosorbent assay ( ELISA ). Schistosoma‐infected patients had significant larger SEA ‐induced granuloma than controls. IL ‐17 (125 pg/mL) induced the optimum size for granuloma within 3‐7 days. However, IL ‐22 at different concentrations up to 300 pg/mL had no effect on granuloma formation. Using both cytokines simultaneously, IL ‐22 suppressed the effect of IL ‐17 and prevented granuloma formation. IL ‐17 significantly decreased TNF ‐α, H 2 O 2 and NO levels in Schistosoma ‐infected individuals. In contrast, IL ‐22 increased TNF ‐α and H 2 O 2 levels. In conclusion, IL ‐17 accelerates SEA ‐induced granuloma formation and inhibits granulocytes functions in Schistosoma ‐infected patients, while IL ‐22 inhibited the granuloma formation, but enhanced granulocyte functions.