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A recombinant chimeric protein composed of human and mice‐specific CD 4 + and CD 8 + T‐cell epitopes protects against visceral leishmaniasis
Author(s) -
Martins V. T.,
Duarte M. C.,
Lage D. P.,
Costa L. E.,
Carvalho A. M. R. S.,
Mendes T. A. O.,
Roatt B. M.,
MenezesSouza D.,
Soto M.,
Coelho E. A. F.
Publication year - 2017
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12359
Subject(s) - epitope , immunogen , biology , isotype , visceral leishmaniasis , leishmania infantum , recombinant dna , immunology , cd8 , immunogenicity , leishmania , virology , antibody , adjuvant , microbiology and biotechnology , leishmaniasis , antigen , parasite hosting , monoclonal antibody , biochemistry , gene , world wide web , computer science
Summary In this study, a recombinant chimeric protein ( RCP ), which was composed of specific CD 4 + and CD 8 + T‐cell epitopes to murine and human haplotypes, was evaluated as an immunogen against Leishmania infantum infection in a murine model. BALB /c mice received saline were immunized with saponin or with RCP with or without an adjuvant. The results showed that RCP /saponin‐vaccinated mice presented significantly higher levels of antileishmanial IFN ‐γ, IL ‐12 and GM ‐ CSF before and after challenge, which were associated with the reduction of IL ‐4 and IL ‐10 mediated responses. These animals showed significant reductions in the parasite burden in all evaluated organs, when both limiting dilution and quantitative real‐time PCR techniques were used. In addition, the protected animals presented higher levels of parasite‐specific nitrite, as well as the presence of anti‐ Leishmania IgG2a isotype antibodies. In conclusion, the RCP /saponin vaccine could be considered as a prophylactic alternative to prevent against VL .