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Protection and immunological study on two tetraspanin‐derived vaccine candidates against schistosomiasis japonicum
Author(s) -
Chen L.,
Chen Y.,
Zhang D.,
Hou M.,
Yang B.,
Zhang F.,
Zhang W.,
Luo X.,
Ji M.,
Wu G.
Publication year - 2016
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12338
Subject(s) - tetraspanin , biology , schistosoma japonicum , antigen , immunogenicity , schistosomiasis , schistosoma mansoni , immunology , schistosoma , immune system , cd81 , thioredoxin , virology , mhc class ii , major histocompatibility complex , cell , helminths , virus , gene , genetics , hepatitis c virus
Summary Tetraspanins ( TSP s) are proteins found on the surface of helminth parasites of the genus Schistosoma and are regarded as potentially protective antigens. The large extracellular loop of Schistosoma mansoni tetraspanin‐2, Sm‐ TSP ‐2, when fused to a thioredoxin partner and formulated with Freund's adjuvants, has been shown to be an efficacious vaccine against murine schistosomiasis. It is well recognized that CD 4 + T‐cell‐dependent immunity might play an important role against schistosomes; however, the contribution of CD 8 + T cells against multicellular pathogen is still uncertain. The exogenous protein‐pulsed dendritic cells (DCs) can easily activate CD 4 + T cells response, while CD 8 + T cells response was relatively difficult to be induced. In this study, we evaluated the immunogenicity of TSP 2 HD antigen (hydrophilic domain of the S. japonicum tetraspanin‐2) and TAT (the protein transduction domain of HIV ‐1)‐coupled TSP 2 HD protein. As TAT ‐fused protein could promote major histocompatibility complex class I‐dependent antigen presentation in vitro , TAT ‐ TSP 2 HD ‐pulsed DC s induced stronger proliferation of schistosome‐specific CD 8 + T cells compared with DC s incubated with TSP 2 HD alone. Vaccination with TAT ‐ TSP 2 HD ‐pulsed DC s in vivo could improve disease outcome in S. japonicum‐ infected mice and was slightly superior to vaccination with DC s treated with TSP 2 HD . In summary, these data showed that TAT fusion proteins could help activate CD 8 + cells and Th1 cells and provide part protection against schistosome.