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Monitoring of local CD 8β‐expressing cell populations during Eimeria tenella infection of naïve and immune chickens
Author(s) -
Wattrang E.,
Thebo P.,
Lundén A.,
Dalgaard T. S.
Publication year - 2016
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12331
Subject(s) - biology , ctl* , immune system , degranulation , eimeria , cytotoxic t cell , immunology , in vitro , immunity , t cell , antigen , t cell receptor , cellular immunity , microbiology and biotechnology , receptor , cd8 , biochemistry
Summary The purpose of this study was to monitor abundance and activation of local CD 8β‐expressing T‐cell populations during Eimeria tenella infections of naïve chickens and chickens immune by previous infections. Chickens were infected with E. tenella up to three times. Caecal T‐cell receptor ( TCR ) γ/δ‐ CD 8β+ cells (cytotoxic T lymphocytes; CTL ) and TCR γ/δ+ CD 8β+ cells were characterized with respect to activation markers (blast transformation, CD 25 and cell surface CD 107a). Cells were also induced to degranulate in vitro as a measure of activation potential. Major findings included a prominent long‐lasting, up to 6 weeks, increase in the proportion of CTL among caecal CD 45+ cells in the later stages after primary E. tenella infection. These CTL also showed clear signs of activation, that is blast transformation and increased in vitro induced degranulation. At second and third E. tenella infection, chickens showed strong protective immunity but discrete signs of cellular activation were observed, for example increased in vitro induced degranulation of CTL . Thus, primary E. tenella infection induced clear recruitment and activation of local CTL . Upon subsequent infections of strongly immune chickens cellular changes were less prominent, possibly due to lower overall numbers of cells being activated because of the severe restriction of parasite replication.