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Effects of peripheral blood mononuclear cells on Haemonchus contortus larval motility in vitro
Author(s) -
Holt R. M.,
Shepherd E. A.,
Ammer A. G.,
Bowdridge S. A.
Publication year - 2015
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12219
Subject(s) - peripheral blood mononuclear cell , haemonchus contortus , biology , motility , andrology , parasite hosting , immunology , incubation , in vitro , microbiology and biotechnology , helminths , biochemistry , medicine , world wide web , computer science
Summary The objective of this experiment was to determine effects of peripheral blood mononuclear cells ( PBMC ), derived from parasite‐resistant St. Croix ( STC ) and parasite‐susceptible Suffolk ( SF ) sheep, on motility of Haemonchus contortus L 3 stage larvae in vitro . Peripheral blood mononuclear cells were collected from 10 lambs of each breed, 5 naïve and 5 which had received a priming infection with H. contortus . Larval motility was quantified using a MIF Nikon Sweptfield microscope and NIS Elements AR software, and measurements included path length ( PL ) (μm), velocity ( VEL ) (μm/s) and acceleration ( ACC ) (μm/s 2 ). After 18 h of incubation, PL and VEL were greatest in larvae cultured with SF ‐derived PMBC and were significantly different from all other groups ( P < 0·01). No difference was observed in PL or VEL between larvae exposed to naïve or primed STC ‐derived PBMC and primed‐ SF PBMC . Differences in ACC were detected between larvae cultured with primed STC ‐derived PBMC (10·91 μm/s 2 ) and naïve SF ‐derived PBMC (45·7 μm/s 2 ) ( P = 0·035). These data indicate an innate ability of STC ‐derived PBMC to severely inhibit larval motility.