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Immunostimulatory potential and proteome profiling of Leishmania donovani soluble exogenous antigens
Author(s) -
Kumar A.,
Samant M.,
Misra P.,
Khare P.,
Sundar S.,
Garg Ravendra,
Dube A.
Publication year - 2015
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12189
Subject(s) - leishmania donovani , biology , proteome , antigen , immune system , visceral leishmaniasis , western blot , leishmaniasis , leishmania , microbiology and biotechnology , immunology , biochemistry , parasite hosting , gene , world wide web , computer science
Summary Isolation of the soluble exogenous antigens ( SEA gs), its immune response study and proteome profiling is an essential prerequisite for understanding the molecular pathogenesis of Leishmania donovani . The immunostimulatory potential of L. donovani SEA gs, purified from culture of L. donovani clinical isolate, was evaluated for their ability to induce cellular responses in treated/cured hamsters. SEA gs induced significant proliferative responses in lymphocytes ( SI 5·6 ± 2·3; P < 0·01) isolated from cured hamster. In addition, significant NO production in response to SEA gs was also noticed in macrophages of hamsters, mouse and human cell lines (J774A‐1 and THP 1). Western blot analyses with antibodies against proteophosphoglycan ( PPG ; surface‐expressed and secreted molecule) of L . donovani revealed that PPG molecules are also present in L. donovani SEA gs. Mass spectrometry ( MS )‐based proteome analysis of 12 protein bands of SEA gs through MALDI ‐ TOF / TOF endorsed the identification of some Th1‐stimulatory immunogenic proteins. These immunogenic proteins may offer increased hope for the discovery of new promising vaccine candidates against visceral leishmaniasis ( VL ). The overall results suggest that immunostimulatory molecules are present in the SEA gs, which may be further exploited, for developing a subunit vaccine against VL a fatal human disease.