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Plasma I g G autoantibody against actin‐related protein 3 in liver fluke O pisthorchis viverrini infection
Author(s) -
Rucksaken R.,
Hao O.,
Pinlaor P.,
Pairojkul C.,
Roytrakul S.,
Yongvanit P.,
Selmi C.,
Pinlaor S.
Publication year - 2015
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12188
Subject(s) - biology , autoantibody , actin , immunology , microbiology and biotechnology , antibody
Summary Opisthorchiasis secondary to O pisthorchis viverrini infection leads to cholangiocellular carcinoma through chronic inflammation of the bile ducts and possibly inducing autoimmunity. It was hypothesized that plasma autoantibodies directed against self‐proteins are biomarkers for opisthorchiasis. Plasma from patients with opisthorchiasis was tested using proteins derived from immortalized cholangiocyte cell lines, and spots reacting with plasma were excised and subjected to LC ‐ MS / MS . Seven protein spots were recognized by I g G autoantibodies, and the highest matching scored protein was actin‐related protein 3 ( ARP 3). The antibody against ARP 3 was tested in plasma from 55 O . viverrini ‐infected patients, 24 patients with others endemic parasitic infections and 17 healthy controls using W estern blot and ELISA . Immunoreactivity against recombinant ARP 3 was significantly more prevalent in opisthorchiasis compared to healthy controls at W estern blotting and ELISA ( P < 0·05). Plasma ARP 3 autoantibody titres were also higher in opisthorchiasis compared to healthy individuals ( P < 0·01) and other parasitic infections including S trongyloides stercoralis ( P < 0·001), echinostome ( P < 0·05), hookworms ( P < 0·001) and T aenia spp. ( P < 0·05). It was further characterized in that the ARP 3 autoantibody titre had a sensitivity of 78·18% and specificity of 100% for opisthorchiasis. In conclusion, it may be suggested that plasma anti‐ ARP 3 might represent a new diagnostic antibody for opisthorchiasis.