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The production and comparative evaluation of native and recombinant antigens for the fast serodiagnosis of cystic echinococcosis with dot immunogold filtration assay
Author(s) -
Chen X.,
Chen X.,
Lu X.,
Feng X.,
Wen H.
Publication year - 2015
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12151
Subject(s) - antigen , recombinant dna , echinococcosis , cystic echinococcosis , biology , immunogold labelling , immunology , serology , immunoassay , antibody , virology , biochemistry , zoology , gene
Summary Clinical diagnosis and post‐surgery assessment of cystic echinococcosis depend on laboratory serodiagnosis and ultrasound examinations. This study aims to produce the recombinant antigen ( rAgB ) and compare its diagnostic effect with natural antigens (crude fluid antigen, protoscolex antigen). After rAgB , crude fluid antigen, protoscolex antigen were produced, and the diagnostic accuracy was evaluated with dot immunogold filtration assay (DIGFA) by the sera from the following groups: surgically confirmed cystic echinococcosis patients ( n = 113), alveolar echinococcosis patients ( n = 46), other parasitic diseases ( n = 49), nonparasitic hepatic diseases ( n = 63) and healthy people ( n = 121). In diagnosing cystic echinococcosis, the sensitivity of recombinant AgB was 77·9% and the specificity was 98·3%. The crude fluid antigen B showed a sensitivity of 92·9% and specificity of 81·0%. The protoscolex antigen had sensitivity of 87·6% and specificity of 90·9%. The recombinant AgB indicates the advantage of no cross‐reaction with other parasite diseases or nonparasite hepatic diseases. Recombinant antigen B can improve the specificity but decrease the sensitivity. The combination of native and recombinant antigens will improve the overall performance of serodiagnosis of cystic echinococcosis.