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Immunological response and protection of mice immunized with plasmid encoding Toxoplasma gondii glycolytic enzyme malate dehydrogenase
Author(s) -
Hassan I. A.,
Wang S.,
Xu L.,
Yan R.,
Song X.,
XiangRui L.
Publication year - 2014
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12146
Subject(s) - biology , toxoplasma gondii , malate dehydrogenase , immunology , immunization , dna vaccination , immunoglobulin e , antibody , cd8 , immune system , toxoplasmosis , titer , antigen , booster dose , virology , enzyme , biochemistry
Summary Toxoplasma gondii Malate dehydrogenase (TgMDH) plays an important role as part of the energy production cycle. In this investigation, immunological changes and protection efficiency of this protein delivered as a DNA vaccine have been evaluated. Mice were intramuscularly immunized with pTgMDH , followed by challenge with virulent T. gondii RH strain, 2 weeks after the booster immunization. Compared to the control groups, the results showed that pTgMDH has stimulated specific humoral response as demonstrated by significant high titers of total IgG and subclasses IgG 1 and IgG 2a , beside IgA and IgM, but not IgE. Analysis of cytokine profiles revealed significant increases of IFN‐γ, IL‐4 and IL‐17, while no significant changes were detected in TGF‐β1. In cell‐mediated response, both T lymphocytes subpopulations CD4 + and CD8 + were positively recruited as significant percentages were recorded in response to immunization with TgMDH. Significant long survival rate, 17 days, has been observed in the TgMDH vaccinated group, in contrast with control groups which died within 8–9 days after challenge. These results demonstrated that TgMDH could induce significant immunological responses leading to a considerable level of protection against acute toxoplasmosis infection.