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Immune response of γδT cells in S chistosome japonicum –infected C57 BL /6 mouse liver
Author(s) -
Xie H.,
Chen D.,
Li L.,
Yu X.,
Wu C.,
Gu H.,
Tang X.,
Peng A.,
Huang J.
Publication year - 2014
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12135
Subject(s) - cd3 , biology , schistosoma japonicum , t cell receptor , il 2 receptor , immunology , immune system , flow cytometry , cd69 , nkg2d , interleukin 21 , t cell , microbiology and biotechnology , cytotoxic t cell , cd8 , in vitro , schistosomiasis , biochemistry , helminths
Summary Systematic evaluation of the role of γδT cells during the S chistosoma japonicum infection has not been reported, despite the fact that γδT cells contribute to many infectious diseases in innate immunity. Therefore, the aim of this study was to observe the properties of γδT cells in the liver of C57 BL /6 mice infected by S . japonicum . In this report, using immuno‐fluorescent histological analysis, γδT cells were found around hepatic granulomatous. Moreover, the flow cytometry results revealed that the percentage of hepatic γδT cells increased significantly after S. japonicum infection. More interestingly, a subset of CD 3 − γδ TCR + cells were found and markedly increased after infection. Furthermore, expression of activation markers ( CD 25 and CD 69) and cytokine profiles were detected in these hepatic CD 3 + γδ TCR + and CD 3 − γδ TCR + cells. The significantly higher level of CD 69, IL ‐4 and IL ‐17 were observed in CD 3 + γδ TCR + cells after infection, suggesting that CD 3 + γδ TCR + cells instead of CD 3 − γδ TCR + cells might play a predominant role during the infection. Finally, our results indicated that the expression of NKG 2D on CD 3 + γδ TCR + cells was higher than that on CD 3 − γδ TCR + cells. Collectively, γδT cells could play an important role in the liver of C57 BL /6 mouse during japonicum infection.