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CD 8α − DC is the major DC subset which mediates inhibition of allergic responses by S chistosoma infection
Author(s) -
Liu J.Y.,
Lu P.,
Hu L.Z.,
Shen Y.J.,
Zhu Y.J.,
Ren J.L.,
Ji W.H.,
Zhang X.Z.,
Wu Z.Q.,
Yang X.Z.,
Yang J.,
Li L.Y.,
Yang X.,
Liu P.M.
Publication year - 2014
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12134
Subject(s) - immunology , cd80 , adoptive cell transfer , biology , cd8 , allergic inflammation , immunoglobulin e , allergy , interleukin 13 , cd86 , mucus , interleukin 4 , cytotoxic t cell , cytokine , antigen , immune system , t cell , cd40 , in vitro , antibody , ecology , biochemistry
Summary Our and others' previous studies have shown that Schistosoma japonicum ( SJ ) infection can inhibit allergic reactions. We recently reported that DC s played an important role in SJ infection‐mediated inhibition of allergy, which was associated with enhanced IL ‐10 and T regulatory cell responses. Here, we further compared the role of CD 8α + DC and CD 8α − DC subsets for the inhibitory effect. We sorted CD 8α + DC ( SJCD 8α + DC ) and CD 8α − DC ( SJCD 8α − DC ) from SJ ‐infected mice and tested their ability to modulate allergic responses in vivo . The data showed that the adoptive transfer of SJCD 8α − DC was much more efficient than SJCD 8α + DC for the suppression of allergic airway eosinophilia, mucus overproduction, antigen‐specific IgE responses, and Th2 cytokines ( IL ‐4 and IL ‐5). More importantly, we found that the transfer of SJCD 8α − DC , but not SJCD 8α + DC , significantly increased IL ‐10 and TGF ‐β production following OVA exposure. As control, the transfer of DC subsets from naïve mice had no significant effect on allergic inflammation. In addition, SJCD 8α‐ DC expressed significantly higher IL ‐10 but lower IL ‐12, CD 80 and CD 86 than SJCD 8α + DC , fitting a tolerogenic phenotype. The results suggest that CD 8α − DC is the predominant DC subset which is involved in the parasitic infection‐mediated inhibition of allergic inflammation and possibly through enhancing immunomodulatory cytokine ( IL ‐10 and TGF ‐β) production.