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Fasciola hepatica tegumental antigens indirectly induce an M2 macrophage‐like phenotype in vivo
Author(s) -
Adams P. N.,
Aldridge A.,
Vukman K. V.,
Donnelly S.,
O'Neill S. M.
Publication year - 2014
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12127
Subject(s) - biology , immune system , immunology , macrophage , fasciola hepatica , in vivo , population , cytokine , interleukin 4 , interleukin 13 , microbiology and biotechnology , in vitro , helminths , medicine , biochemistry , environmental health
Summary The M2 subset of macrophages has a critical role to play in host tissue repair, tissue fibrosis and modulation of adaptive immunity during helminth infection. Infection with the helminth, Fasciola hepatica, is associated with M2 macrophages in its mammalian host, and this response is mimicked by its excretory‐secretory products (Fh ES ). The tegumental coat of F. hepatica (FhTeg) is another major source of immune‐modulatory molecules; we have previously shown that FhTeg can modulate the activity of both dendritic cells and mast cells inhibiting their ability to prime a Th1 immune response. Here, we report that FhTeg does not induce Th2 immune responses but can induce M2‐like phenotype in vivo that modulates cytokine production from CD 4 + cells in response to anti‐ CD 3 stimulation. FhTeg induces a RELM α expressing macrophage population in vitro, while in vivo, the expression of Arg1 and Ym‐1/2 but not RELM α in FhTeg‐stimulated macrophages was STAT 6 dependent. To support this finding, FhTeg induces RELM α expression in vivo prior to the induction of IL ‐13. FhTeg can induce IL ‐13‐producing peritoneal macrophages following intraperitoneal injection This study highlights the important role of FhTeg as an immune‐modulatory source during F. hepatica infection and sheds further light on helminth–macrophage interactions.