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Giardia duodenalis stimulates partial maturation of bovine dendritic cells associated with altered cytokine secretion and induction of T‐cell proliferation
Author(s) -
Grit G. H.,
Devriendt B.,
Van Coppernolle S.,
Geurden T.,
Hope J.,
Vercruysse J.,
Cox E.,
Geldhof P.,
Claerebout E.
Publication year - 2014
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12095
Subject(s) - biology , cd80 , dendritic cell , immunology , cd40 , immune system , t cell , microbiology and biotechnology , antigen presenting cell , cd8 , secretion , cytotoxic t cell , endocrinology , in vitro , biochemistry
Summary Giardia duodenalis is an important intestinal parasite in animals and humans. The role of dendritic cells ( DC ) in the initiation of the immune response against G. duodenalis is poorly documented. The aim of this study was to test the hypothesis that G. duodenalis interferes with bovine DC function. Therefore, the effect of trophozoites and excretion/secretion products on bovine monocyte‐derived dendritic cells (Mo DC ) was investigated. We assessed Mo DC maturation and cytokine production of G. duodenalis ‐stimulated Mo DC and the ability of these Mo DC to take up antigen and induce lymphocyte proliferation. Little or no upregulation of maturation markers CD 40 and CD 80 was measured, but MHCII expression was increased after stimulation with low parasite concentrations. A dose‐dependent decrease in ovalbumin uptake was observed in G. duodenalis ‐stimulated Mo DC . In addition, stimulated Mo DC induced proliferation of CD 3 − , γδ‐T‐cells and TCR αβ + CD 4 + and CD 8 + T‐cells. Increased transcription of TGF ‐β was shown in CD 4 + T cells, and increased TNF ‐α, TGF ‐β, IL ‐10 and IL ‐4 were seen in γδ‐T‐cells. We found no evidence that G. duodenalis has a regulatory or inhibitory effect on bovine Mo DC . Mo DC stimulated with G. duodenalis are functionally active and able to induce proliferation of T cells that produce both pro‐ and anti‐inflammatory cytokines.