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Analysis of the mucosal immune responses induced by single and trickle infections with the bovine abomasal nematode O stertagia ostertagi
Author(s) -
Mihi B.,
Meulder F.,
Vancoppernolle S.,
Rinaldi M.,
Chiers K.,
Broeck W.,
Goddeeris B. M.,
Vercruysse J.,
Claerebout E.,
Geldhof P.
Publication year - 2014
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12094
Subject(s) - ostertagia ostertagi , biology , downregulation and upregulation , immunology , granulysin , immune system , eosinophilia , granzyme b , granzyme , cytokine , teladorsagia circumcincta , interleukin 10 , perforin , t cell , helminths , cd8 , haemonchus contortus , gene , biochemistry
Summary The purpose of this study was to provide more information on the kinetics of the immunological changes occurring in the abomasal mucosa after single and trickle infections with the bovine parasite O stertagia ostertagi . The time course analysis of gene expression revealed that the major changes coincided with the emergence of adult worms from the gastric glands. These changes consisted of a simultaneous upregulation of T h1‐ and T h2‐type cytokines. In addition, a single O . ostertagi infection elicited an upregulation of the epithelial‐derived cytokine IL 33 , while TSLP expression levels were not impacted. Apart from the massive increase in inflammatory cytokines IL 6 , IL 17 and IL 21 , O . ostertagi infection also elicited an upregulation of the immunosuppressors TGFB , IL 10 and ARG 1, as well as NK and γδ‐ T cell markers. Furthermore, the cytotoxic factors granulysin, perforin and granzyme B were upregulated following an O . ostertagi infection. Analysis of cytokine transcript levels in animals receiving trickle infections for 60 days showed a similar trend as observed following a single infection except for IL 33 , IL 6 , GATA ‐3 , TBX 21 and NCR 1 , which were no longer upregulated after trickle infections. Finally, the long trickle infections were associated with mucosal eosinophilia and mastocytosis.