Premium
Cytokine m RNA expression in bronchoalveolar lavage cells during D ictyocaulus viviparus infection in calves
Author(s) -
Holmgren S.,
Hagberg Gustavsson M.,
Lundén A.,
Wattrang E.
Publication year - 2014
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12083
Subject(s) - bronchoalveolar lavage , biology , immunology , immune system , cytokine , immunity , innate immune system , lung , medicine
Summary The purpose of this study was to monitor local cytokine responses to Dictyocaulus viviparus in calves during primary infection and re‐infection. Bronchoalveolar lavage fluid (BALF) was collected weekly from experimentally infected calves and interleukin‐2 (IL‐2), IL‐4, IL‐5, IL‐10 and IFN‐γ m RNA expression was quantified in BALF cells. The major finding was a prominent transient increase in IL‐4 m RNA expression, compared with that of uninfected calves, observed in BALF cells collected 2–3 weeks post‐primary D. viviparus infection. At 2 weeks post‐infection, macroscopic worms were also first observed in BALF. Calves re‐infected after 10 weeks were partially immune which was evident at slaughter 5 weeks post‐infection as a lower worm burden than in previously naïve calves infected at the same time. IL‐4 m RNA expression in BALF cells 2 weeks post‐re‐infection was increased compared with that of uninfected animals but not as high as that of primarily infected calves. BALF cell expression of the other cytokines tested for was not as clearly effected by the D. viviparus infection. It seems likely that the strong IL‐4 response observed during primary infection reflects an innate response to the worms that may initiate an ensuing Th2 response, which confers protective immunity.