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Differential IFN ‐γ production by adult and neonatal blood CD 56 + natural killer ( NK ) and NK ‐like‐ T cells in response to T rypanosoma cruzi and IL ‐15
Author(s) -
Guilmot A.,
Carlier Y.,
Truyens C.
Publication year - 2014
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/pim.12077
Subject(s) - biology , immunology , immune system , interferon gamma , innate immune system , interleukin 12 , cd16 , interleukin 21 , flow cytometry , cd3 , natural killer cell , nkg2d , interferon , cd8 , cytotoxic t cell , in vitro , biochemistry
Summary Early interferon‐gamma ( IFN ‐γ) release by innate cells is critical to direct type 1 immune response able to control intracellular pathogens like T rypanosoma cruzi . Although CD 56 bright natural killer ( NK ) cells are reported to be potent early IFN ‐γ producers, other CD 56 + cells like CD 56 dim NK cells and NK ‐like T cells have recently been shown to also release IFN ‐γ. We have here studied the contribution of each CD 56 + lymphocyte populations in early IFN ‐γ production in both adults and neonates. On this purpose, we analysed the kinetics of IFN ‐γ production by RT ‐ PCR , ELISA and flow cytometry from 2 h onwards after T . cruzi and IL ‐15 stimulation and sought for the responding CD 56 + cells. CD 56 bright and CD 56 dim CD 16 − NK cells were the more potent IFN ‐γ early producers in response to IL ‐15 and parasites in adults and neonates. In both age groups, the majority of IFN ‐γ producing cells were NK cells. However, on the contrary to neonates, CD 3 + CD 56 + NK ‐like T cells and CD 3 + CD 56 − ‘classical’ T cells also contributed to early IFN ‐γ production in adults. Altogether, our results support that whereas NK cells responded almost similarly in neonates and adults, cord blood innate CD 56 + and CD 56 − T cells displayed major quantitative and qualitative defects that could contribute to the well‐known neonatal immune immaturity.